Extended Data

• Description: This file contains the image of patched colonies from the YPD plating of the patch that was incubated at 34°C for five days. . Resource Type: Image. DOI: 10.22002/yr5hv-zzv60
• Description: This file contains the records of transfers and temperatures over the course of evolution.. Resource Type: Dataset. DOI: 10.22002/shdef-nkk25
• Description: This file contains the list of genomic features that are duplicated in the evolved clone. . Resource Type: Dataset. DOI: 10.22002/w1aw9-64n31
• Description: This is the vcf file containing all variants called in the evolved clone that are not present in the ancestor along with their annotations. . Resource Type: Dataset. DOI: 10.22002/4a6hn-ehr12
• Description: This file contains a summary of the disruptive gene variants identified in the evolved clone.. Resource Type: Dataset. DOI: 10.22002/8z8pn-b1e39

Author Contributions

• Emery R. Longan: Conceptualization, Investigation, Writing - original draft, Writing - review & editing
• Justin C. Fay: Funding acquisition, Writing - original draft, Writing - review & editing

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