µP

microPublication Biology

Florian Rouaud1, Isabelle Mean1, Lionel Jond1, and Sandra Citi1§ORCID logo

1Department of Molecular and Cellular Biology, University of Geneva

§Correspondence to: Sandra Citi (sandra.citi@unige.ch)

Abstract

Description

Methods

Extended Data

  • Description: Extended Data Figure Legend: A) Validation of Crispr/Cas9-mediated deletion of CGNL1 in two distinct clonal lines of bEnd.3 cells by genomic sequencing. The WT sequence is shown on top. B) Validation of KO by immunoblotting analysis of the expression of CGNL1, NM2A, NM2B and ZO-1 in WT and CGNL1-KO bEnd.3 cells. Beta-tubulin was used as a loading control. Numbers on the left refer to migration of molecular weight standards.. Resource Type: Image. DOI: 10.22002/mp0ys-6b272

Acknowledgements

Funding

Author Contributions

  • Florian Rouaud: Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization
  • Isabelle Mean: Methodology
  • Lionel Jond: Methodology
  • Sandra Citi: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Validation, Writing - original draft, Writing - review & editing

Reviewed By

Anonymous

History

  • Received: 4/14/2025
  • Revision Received: 6/24/2025
  • Accepted: 6/23/2025
  • Published Online: 6/24/2025

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Copyright

© 2025 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

microPublication Biology is published by

1200 E. California Blvd. MC 1-43 Pasadena, CA 91125

The microPublication project is supported by

The National Institute of Health -- Grant #: 1U01LM012672-01

microPublication Biology:ISSN: 2578-9430