µP

microPublication Biology

Stephanie L. Sarbanes1§ORCID logo, J. Robert Hogg2ORCID logo, and Antonina Roll-Mecak1,2§ORCID logo

1National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, United States

2Biochemistry & Biophysics Center, National Heart Lung and Blood Institute, Bethesda, Maryland, United States

§Correspondence to: Stephanie L. Sarbanes (sarbanessl@nih.gov) Antonina Roll-Mecak (antonina@ninds.nih.gov)

Abstract

Description

Methods

Reagents

Extended Data

  • Description: Validation of siRNA knock-down efficiency for RNA decay and CCR4-NOT complex factors. RT-qPCR for each gene-of-interest to assess degree of knock-down relative to a NT control siRNA at time of colchicine treatment, 72 hrs post-transfection. Data are graphed as the fold change relative to the NT control condition for each siRNA KD (preceded by normalization to housekeeping gene GAPDH). . Resource Type: Image. DOI: 10.22002/2j04v-t2c81

Acknowledgements

Funding

Author Contributions

  • Stephanie L. Sarbanes: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review & editing, Visualization
  • J. Robert Hogg: Conceptualization, Methodology, Resources, Supervision, Writing - review & editing
  • Antonina Roll-Mecak: Conceptualization, Funding acquisition, Resources, Supervision, Writing - review & editing

Reviewed By

Anonymous

History

  • Received: 9/30/2025
  • Revision Received: 10/21/2025
  • Accepted: 10/21/2025
  • Published Online: 11/18/2025

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Copyright

© 2025 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

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The microPublication project is supported by

The National Institute of Health -- Grant #: 1U01LM012672-01

microPublication Biology:ISSN: 2578-9430