{ "componentChunkName": "component---src-templates-article-page-js", "path": "/journals/biology/W2H59W", "result": {"data":{"micropubApp":{"manuscript":{"id":"6cf5f2b3-489a-4f23-b04a-348c44ee7363","submissionTypes":["Commodity Validation","New Finding"],"doi":"10.17912/W2H59W","pmcId":"7255870","pmId":"32550351","proteopedia":null,"reviewPanel":null,"species":["C. elegans"],"corrections":null,"history":{"received":"2016-12-12T00:00:00.000Z","revisionReceived":null,"accepted":"2017-01-27T08:00:00.000Z","published":"2017-02-08T08:00:00.000Z","indexed":null},"versions":[{"id":"4c578747-8d6c-4de3-b0c8-f78b6c7cc714","decision":"publish","abstract":null,"acknowledgements":null,"authors":[{"affiliations":["NemaMetrix, Inc,. 44 W 7th Ave., Eugene, OR 97401 USA."],"credit":[""],"email":"terra.hiebert@nemametrix.com","firstName":"Terra","lastName":"Hiebert","submittingAuthor":true,"correspondingAuthor":true,"equalContribution":false,"WBId":null,"orcid":null},{"affiliations":["NemaMetrix, Inc,. 44 W 7th Ave., Eugene, OR 97401 USA."],"credit":[""],"email":"","firstName":"Adela","lastName":"Chicas-Cruz","submittingAuthor":false,"correspondingAuthor":false,"equalContribution":false,"WBId":null,"orcid":null},{"affiliations":["NemaMetrix, Inc,. 44 W 7th Ave., Eugene, OR 97401 USA."],"credit":[""],"email":"","firstName":"Kathyrn","lastName":"McCormick","submittingAuthor":false,"correspondingAuthor":false,"equalContribution":false,"WBId":null,"orcid":null}],"extendedData":null,"funding":"Oregon Nanoscience and Microtechnologies Institute (ONAMI) and NemaMetrix, Inc.","image":{"url":"https://portal.micropublication.org/uploads/W2H59W.jpg"},"imageCaption":"","imageTitle":"","methods":null,"reagents":"

Strains:  MT8944: mod-5(n822) I; MT9772: mod-5(n3314) I
\nControl Strain: N2

\n","patternDescription":"

In C. elegans, the reuptake of serotonin (5-HT) is facilitated by mod-5, which encodes a 5-HT transporter that is orthologous to a human 5-HT transporter (SLC6A4).  mod-5 has been shown to effect both feeding and locomotion in C. elegans (Ranganathan et al., 2001; Jafarau et al. 2011).  We obtained and analyzed EPG data using a microfluidic device (NemaMetrix) for mod-5 null mutant strains, mod-5(n3314) (A, Exp 1 n=15; Exp 2 n=32) and mod-5(n822), (B, Exp 1 n=17; Exp 2 n=27) and N2 control worms (A, n=16 and 31; B, n=18 and 27 for Exp 1 and 2, respectively) in M9 saline buffer (2-minute recording duration). Mutations to the C. elegans serotonin reuptake transporter, mod-5, lead to an accumulation of serotonin at the synaptic cleft, which results in a significant increase in baseline pharyngeal pumping frequency in three out of four experiments (A, N2=0.10 ± 0.04 and 0.09 ± 0.03 Hz ; mod-5(n3314)=1.41 ± 0.44 and 2.26 ± 0.13 Hz; B, N2=0.98± 0.42 and 0.44 ± 0.08; mod-5(n822)=1.64 ± 0.33 and 3.76 ± 0.19 Hz; **p<0.005, ***p<0.0001, 2-tailed students t-test).

\n","references":[{"reference":"Ranganathan, R., Sawin, ER, Trent, C., Horvitz, HR (2001) Mutation in the Caenorhabditis elegans serotonin reuptake transporter mod-5 reveal serotonin-dependent and independent activities of fluoxetine. J Neurosci 21(16): 5871-5884.","pubmedId":"11487610","doi":""},{"reference":"Jafarau, D, Xie, Y, Kullyev, A, Liang, B, Sze, JY (2011) Regulation of extrasynaptic 5-HT SERT function in 5-HT absorbing neurons underscores adaptation behavior in C. elegans. J Neurosci 31(24): 8948-8957.","pubmedId":"21677178","doi":""}],"title":"Basal pharyngeal pumping elevated in C. elegans mod-5 mutants","reviews":[{"reviewer":{"displayName":"Veena Prahlad"},"openAcknowledgement":true,"status":{"submitted":true}}]}]}}},"pageContext":{"id":"6cf5f2b3-489a-4f23-b04a-348c44ee7363"}}, "staticQueryHashes": ["1993668605"]}