µP

microPublication Biology

Dagem Yilma Chernet1*, Levi Klassen1*, Sonya Goertzen1, and Juan Nicolas Malagon1§

1Canadian Mennonite University, 500 Shaftesbury Blvd, Winnipeg, MB, Canada R3P 2N2

§Correspondence to: Juan Nicolas Malagon (NMalagon@cmu.ca)

* Authors have equal contribution

Abstract

Description

Methods

Request a detailed protocol

Reagents

Extended Data

  • Description: Movie 1. 2-Dimensional confocal projections showing circulatory tumors cells (CTCs) during early Drosophila pupal leg and wing development. Schematic (A) and 2D projections (B-D) show the movement of CTCs during morphogenesis. Panel A shows schematically the region imaged (dotted line). Panel B shows only the green channel, where invasive tumor cells are marked with GFP. Panel C shows a composite of the green and DIC channels. This panel, in addition to showing tumor cells, also allows for observing the developing legs and wings. Panel D shows the composite of the green and DIC as well as various labels that allow for the direction of CTCs to be observed. CTCs are labelled within circles of various colors. Arrows indicate the direction of CTC movement. Sections of developing leg are labelled with blue lines. The distal boundaries of leg and wing imaginal discs are labelled with yellow lines.. Resource Type: Dataset. DOI: 10.22002/D1.20190
  • Description: Movie 2. 2-Dimensional confocal projections showing circulatory tumors cells (CTCs) during early Drosophila pupal leg and wing development. 2D projections (A-B) showing the movement of CTCs during morphogenesis. Panel A shows only the green channel, where invasive tumor cells are marked with GFP. Panel C shows a composite of the green and DIC channel. CTCs are labelled within circles of various colors. Arrows indicate the direction of CTC movement. . Resource Type: Dataset. DOI: 10.22002/D1.20191
  • Description: Movie 3. 2-Dimensional confocal projections showing circulatory tumors cells (CTCs) during early Drosophila pupal leg and wing development. 2D projections (A-B) showing the movement of CTCs during morphogenesis. Panel A shows only the green channel, where invasive tumor cells are marked with GFP. Panel C shows a composite of the green and DIC channel. CTCs are labelled within circles of various colors. Arrows indicate the direction of CTC movement. . Resource Type: Dataset. DOI: 10.22002/D1.20192

Acknowledgements

Funding

This work was supported by Canadian Mennonite University.

Author Contributions

  • Dagem Yilma Chernet: Conceptualization, Investigation, Writing - original draft, Writing - review & editing, Data curation, Formal analysis
  • Levi Klassen: Data curation, Formal analysis, Methodology, Writing - original draft, Writing - review & editing, Investigation
  • Sonya Goertzen: Investigation, Data curation, Formal analysis
  • Juan Nicolas Malagon: Data curation, Formal analysis, Writing - review & editing, Investigation, Supervision, Funding acquisition

Reviewed By

Jacob Kagey

History

  • Received: 11/3/2021
  • Revision Received: 5/30/2022
  • Accepted: 6/9/2022
  • Published Online: 6/10/2022
  • Indexed: 6/23/2022

References

Copyright

© 2022 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

PubMed Central: 9242741

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microPublication Biology:ISSN: 2578-9430