µP

microPublication Biology

Jack R. Gregory1,2,3ORCID logo, Nicholas J. Ricottilli1ORCID logo, and Jian-Qiu Wu1§ORCID logo

1Department of Molecular Genetics, The Ohio State University, Columbus, Ohio, United States

2Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University, Columbus, Ohio, United States

3Cellular, Molecular, and Biochemical Sciences Program, The Ohio State University, Columbus, Ohio, United States

§Correspondence to: Jian-Qiu Wu (wu.620@osu.edu)

Abstract

Description

Methods

Reagents

Extended Data

  • Description: Movie 1. Time-lapse microscopy of the septin Spn4 in 81nmt1-mEGFP-spn4 and 81nmt1-GFP(S65T)-spn4 strains. The indicated strains were grown in YE5S for ~12 h, and then were washed 3x with EMM5S and grown exponentially in EMM5S for ~24 h at 25℃. These strains were imaged on an EMM5S + gelatin pad using a spinning disk confocal microscope every 30 sec for 1 h. Maximal intensity projection of 5 slices with 2 µm spacing are shown. 7 frames per second.. Resource Type: Audiovisual. DOI: 10.22002/e4bh4-a6g30

Acknowledgements

Funding

Author Contributions

  • Jack R. Gregory: Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Visualization, Project administration, Writing - original draft, Writing - review & editing
  • Nicholas J. Ricottilli: Investigation, Methodology, Writing - review & editing
  • Jian-Qiu Wu: Conceptualization, Supervision, Resources, Funding acquisition, Writing - review & editing

Reviewed By

Anonymous

Nomenclature Validated By

Anonymous

History

  • Received: 11/10/2025
  • Revision Received: 11/22/2025
  • Accepted: 11/24/2025
  • Published Online: 11/26/2025

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Copyright

© 2025 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

microPublication Biology is published by

1200 E. California Blvd. MC 1-43 Pasadena, CA 91125

The microPublication project is supported by

The National Institute of Health -- Grant #: 1U01LM012672-01

microPublication Biology:ISSN: 2578-9430